Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bcabased protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. Although bicinchoninic acid bca has been widely used to determine protein concentration, the mechanism of interaction between protein, copper ion and bca in this assay is still not well known. Number description 23225 bca protein assay kit, sufficient reagents for 500 test tube or 5,000 microplate assays 23227 bca protein assay kit, sufficient reagents for 250 test tube or 2,500 microplate assays kit contents. Thermo scientific pierce protein assay technical handbook.
Using a pierce bca assay kit for whole protein quantification, our lower end of the curve is not sensitive to picking up even our standards for 25 gml. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Procedure for the pierce microplate bca protein assay kitreducing agent compatible. Pierce microplate bca protein assay kit fisher scientific. Kit provides a convenient procedure for quantifying protein, and the assay protocol. Thermo scientific pierce microplate bca protein assay kit. If they can be stored and reused to create another standard curve, what is recommended for storage temp. Thermo scientific pierce 660nm protein assay overview highlights typical response curves 14 bca based protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca protein assay reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. Bca assay in situations in which it is normally unable to be read. An adaptation of the traditional bca assay, the rapid gold bca protein assay kit. Bsa bovine serum albumin is used as the standard and samples are. The principle of lowry assay, biuret assay, and bicinconinich bca assay protein assays duration.
This reducing agentcompatible rac version of the thermo scientific pierce bca protein assay provides accurate determination of protein concentration in samples that contain dithiothreitol dtt, 2mercaptoethanol bme, tcep and other disulfide reducing agents. Here, we illustrate how the spectramax id5 reader, in combination with softmax pro software, is used to quantitate a cellular protein sample with two different kits, pierce bca protein assay kit and pierce rapid gold bca protein assay kit. The thermo scientific pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Bca protein assay introduction the glomaxmulti jr and the pierce bca protein assay provide a simple method for quantifying protein over a large range. Jun 19, 2018 the principle of lowry assay, biuret assay, and bicinconinich bca assay protein assays duration. Certain substances interfere with the pierce bca assay kit. Incompatible substances amount compatible buffer systems nacetylglucosamine 10 mm in pbs, ph 7. Streamline bcabased protein quantitation molecular devices. Thermo scientific pierce bca protein assay kit protein. The bca is used for the same reasons the lowry is used. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional. I have found the assay to be fairly sensitive and reliable, however a few points should be noted. The bicinchoninic acid assay bca assay is a biochemical assay for determining the total level of protein in a solution, similar.
If precise quantitation of an unknown protein is required, it is advisable to select a protein. Pierce bca reagents provide accurate determination of protein concentration with most sample types encountered in protein research. No action shall be taken involving any personal risk or without suitable training. Pierce bca protein assay kit university of arizona. Using the glomax discover system with the pierce bca protein assay. Pierce bca protein assay kit is a twocomponent, highprecision, detergent compatible.
The quantipro bca assay kit is based on the same principals as the bca1 kit, except the quantipro bca kit gives a linear response from 0. Pierce bca reagents pierce bca protein assay reagent b. Using the micro bca protein assay kit pierce company. Standard protein quantification methods, such as bicinchoninic acid assay bca will suffice. Pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible protein assay for determination of protein concentration.
Bca protein assay kit from thermo scientific pierce. This was done by analyzing the precision, accuracy, linearity and range of the method. When ready to run the assay, equal amounts of protein should be loaded per condition. Piercea protein assay kit bc thermo fisher scientific. Thermo scientific pierce bca protein assay kit fisher. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bca based protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. Dilution scheme for standard test tube protocol and microplate procedure. Bicinchoninic acid protein assay kit catalog numbers bca1 and b9643 technical bulletin synonym. Pierce bca reagents provide accurate determination of protein concentration with most sample. Measuring protein concentration using the bca protein assay with. The pierce bca assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications. Procedure for the reducing agentcompatible bca assay. Introduction the modulus by turner biosystems and the bca protein assay kit from pierce provides a simple method for quantifying protein over a large range. Bca product description protein determination is one of the most common operations performed in biochemical research.
If you are not satisfied with the performance of a pierce product, please contact pierce or your local distributor. Mar 27, 2020 prepared from the protein and assayed alongside the unknowns before the concentration of each unknown is determined based on the standard curve. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional differences, providing greater proteintoprotein uniformity. An adaptation of pierces bca protein assay reagent kit product no. The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein.
Other means of bca protein assay reagent a identification. Pierce offers seven colorimetric assays for detection and quantitation of total protein. Bca protein assay kit ab102536 provides a simple, rapid, detergent tolerant up to 5% procedure for determining the concentration of proteins in solution. Hazardous thermal decomposition products specific hazards arising from the chemical. Here we describe the use of the pierce rapid gold bca and pierce detergent compatible bradford assays to quantitate total protein in cell lysates. Competitive binding to cuprous ions of protein and bca in the. The thermo scientific pierce bca protein assay kit is a.
Studies of the bca assay for determination of total protein. The bicinchoninic acid bca assay is available in kit form from pierce rockford, ill. This is the protocol to be used in the determination of total protein in brain tissue by the pierce bca method. Bicinchoninic acid assay an overview sciencedirect topics. If the total protein concentration in the samples is high 2,000. Dc protein assay instruction manual for technical service call your local biorad office or in the u. The principle of the bicinchoninicacid bca assay is similar to the lowry procedure,1 in that both rely on the. Safety data sheet chandlergilbert community college. Pierce bca protein assay reagent a safety data sheet product name pierce bca protein assay reagent a conforms to regulation ec no. Both assays were conducted according to the manufacturers protocols, in a microplate format. The thermo scientific pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible assay reagent set to measure a562nm total protein concentration compared to a protein standard. Oct 22, 2007 the bca protein assay kit from thermo scientific pierce product no.
Bicinchoninic acid bca protein assay smith rice university. This protocol makes use of thermo scientifics bca protein assay in a 96well tissue culture plate format to measure the amount of protein in small volumes of sample. Pierce bca protein assay kit, sufficient reagents for 500 testtube or 5000. Tools and reagents for improved quantitation of total or. The bca protein assay is a popular method for colorimetric detection and quantitation of total protein. Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater proteintoprotein uniformity. Pierce bca protein assay kit thermo fisher scientific. Thermo scientific pierce bca protein assay reagent a. Smith at the pierce chemical company, now part of thermo fisher scientific, is a biochemical assay for determining the total concentration of protein in a solution 0. The bca protein assay kit from thermo scientific pierce product no. Herein, we used the pierce bca protein assay kit thermo scientific, catalog no. This watersoluble complex exhibits a strong absorbance at 562 nm that is.
Studies of the bca assay for determination of total protein in allergen extracts title swedish abstract the aim of this thesis has been to evaluate and to optimize the bca protein assay for quantification of total protein. The bicinchoninic acid assay bca assay, also known as the smith assay, after its inventor, paul k. This procedure is very applicable to microtiter plate methods. Bca bicinchoninic acid bsa bovine serum albumin ci confidence interval dpbs dulbeccos pbs id identification rt room temperature sds safety data sheets. Pierce bca protein assay kit, sufficient reagents for 500 testtube or 5000 microplate assays. Measure total protein in cell lysates with spectramax abs. Compatibility chart for bca kit the amount listed is the maximum amount of material allowed in the protein sample without causing a noticeable interference, when 20 ul protein sample is used for bca assay as described in the standard assay. Protein concentration determination in lysates using the standard pierce bca protein assay and pierce rapid gold bca protein assay. The pierce micro bca protein assay reagent kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein.
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